... III 柱的洗涤 IV 分析物的洗脱 Mediwax固相萃取装置（SPE Manifolds)有12、24管和相应吹扫装置（Dry Attachment)可供选择 ...
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这使得定量洗脱四种分析物中的三种产生一个尖锐峰。
This allows quantitative elution of three of the four analytes as one sharp peak.
结果：综合分析多靶点活性，40%乙醇洗脱物和中间层的活性最强。
Result: comprehensive analysis of multi-target results, the comprehensive activities of 40% ethanol elution and the middle layer were the highest.
洗脱物经预柱脱盐和浓缩后进入毛细管反相柱进行反相分离，分离后的肽段直接进入质谱仪离子源进行一级和二级质谱的分析。
Following desalinisation the eluted peptides were further purified by reversed-phase capillary column(150 mm 75 m) and online analysed by the coupled tandem mass spectrometry.
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