分子信标探针(Molecular beacons probe) 可形成发夹结构的寡核苷酸探针，5′末端标记荧光素（EDANS)，3′末端标记淬灭剂(DABCYL)，探针噜扑环与目的DNA 碱基互补，噜扑环两侧为与目的DNA 无关的碱基互补的臂。当探针与靶序列相连，淬灭基团与报告基团逐渐远离，遂发出荧光。
分子信标是一种高灵敏度、高特异性的新型荧光核酸探针。它在与互补DNA或RNA靶序列杂交时放出荧光。
Molecular beacon is a kind of high sensitive and selective fluorescence oligonucleotide probes that is producing fluorescence upon hybridizing with their complementary DNA or RNA targets.
目的：探讨分子信标荧光探针检测结核分枝杆菌的临床应用价值。
Objective To evaluate the molecular beacon in the clinical detection of Mycobacterium tuberculosis.
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