其中，绝大多数藻类是采用两步冰冻法保存。
Most of them were preserved by the two_step freezing method.
水稻组织培养物超低温保存的效果常受到材料特性、冰冻保护剂、降温冰冻法、后处理及恢复培养等因素的影响。
Cryopreservation of tissue cultures of rice is usually influenced by some factors, such as material properties, cryoprotectant, freezing procedure, treatment after freezing and renewing culture.
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