...中,顶端外胚层帽下的骨细胞、软骨细胞、成纤维细胞、肌细胞、神经细胞发生去分化作用,构 成再生胚基 ( regeneration blastema )的形成,继续增殖、分化形成肢体结构。
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利用水稻成熟胚为外植体,接种在NB培养基上,分别附加不同的外源激素,以诱导愈伤组织并促其分化,最终获得水稻再生植株。
The mature embryos were cultivated on NB media with different hormones, by using the mature embryos in rice as explants, and regeneration plants were obtained successfully.
全量培养基比半量培养基更适合愈伤组织继代;MS基本培养基有利于种胚植株再生。
Full quality of basic media were more suitable to callus subculture than half quality of that. But MS basic medium was advantageous to plant regeneration of mature embryo.
胚性愈伤诱导率和植株再生率与培养基的种类、培养基中2,4 - D的浓度、小麦基因型及诱导和分化培养基中激素种类和浓度等有关。
Callus formation frequency as well as plantlet regeneration frequency was dependent on the composition of basal medium and the concentration of 2, 4-d.
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