Based on color complementary,and HPLC analysis of metabolites,we confirmed that the CarRA protein activity detection system was reliable. Our study provides a screening model for specific mutation of lycopene cyclase without affecting phytoene synthase activity.
通过颜色互补的初步鉴定,再以HPLC对代谢产物进行分析,明确了实验中的CarRA蛋白功能活性检测系统的可靠性,为定点突变番茄红素环化酶活性位点而不影响八氢番茄红素合成酶功能活性提供了筛选模型。
参考来源 - 重叠延伸PCR克隆三孢布拉霉carRA基因及其功能活性检测系统的构建·2,447,543篇论文数据,部分数据来源于NoteExpress
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