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成功获得了人BMP4的全长基因，且序列与已知序列高度一致。
The full-length of human BMP4 is obtained successfully and its sequence is highly consistent with the known sequence.
建立一种用于克隆全长基因的、限制性内切酶介导的重叠延伸法 。
The overlap extension mediated by restriction endonuclease to obtain the full length gene was established.
目的：克隆大鼠神经营养因子4全长基因，构建真核细胞表达质粒。
AIM: to clone rat neurotrophin-4 (NT-4) total gene and construct expression plasmid for prokaryotic cells.
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