方法24只新西兰兔分为实验组(12只)和对照组(12只),分离培养实验组兔骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs),Brdu标记,建立兔后肢缺血动物模型,将取于自体的骨髓间充质干细胞制成悬液注射于实验组兔缺血部位,...
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目的在体外成功分离培养标记兔骨髓间充质干细胞(Bone mesenchymal stem cells,BMSCs)的基础上,观察体外冲击波疗法(Extracorporeal sh ..
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...nt 化成骨及成脂的实验研究目的绿色荧光蛋白(green fluorescent protein，GFP)标记兔骨髓间充质干细胞(mesenchymal stem cells，MSCs)，观察其诱导成骨及成脂多向分化的潜能。
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...导兔骨髓间充质干细胞向软骨细胞分化的实验研究 目的探讨兔骨髓间充质干细胞（bone marrow derived mesenchymal stem cells，BMSCs）在特定培养条件下向软骨细胞定向分化的情况。
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目的：观察兔骨髓间充质干细胞的生长特点及其在诱导条件下的成骨能力。
OBJECTIVE To observe the growth characteristics of rabbit bone marrow-derived mesenchymal stem cells and their osteogenic potential under the induced condition.
方法体外培养获取生长状态良好的兔骨髓间充质干细胞，进行成软骨诱导，观察并检测其生物学特性。
Methods Rabbit BMSCs were cultured in vitro induced to chondrocytes, and then the biologic characters of the chondrocyte were observed and tested.
目的：拟建立一套体外分离培养及大量扩增兔骨髓间充质干细胞的方法，并对细胞生物学特性进行观察。
OBJECTIVE: To establish a method for in vitro isolation and cultivation of BMSCs from rabbits and to study their biological characteristics.
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