人胎盘组织造血干/祖细胞的分离富集 关键词】 CD34抗原；造血干细胞；胎盘；免疫磁珠细胞分选；脐血 [gap=1741]Key wordsCD34 antigen; hematopoietic stem cell; placenta; MACS; umbilical cord blood
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利用免疫磁珠细胞分选 magnetic bead cell sorting ; MACS
目前国外采用较多的有荧光激活细胞分选法、磁活化细胞分选法、免疫磁珠分离法、密度梯度离心法。
Various methods of enrichment exit:fluorescent activated cell sorting, magnetic-activated cell sorting, immunomagnetic beads, density gradient.
免疫磁珠分选脐血cd 34 +细胞，接种于各培养体系，无外源性细胞因子条件下进行培养。
CD34 + cells were isolated from fresh umbilical cord blood samples with immunomagnetic beads and seeded into the models to culture ex vivo without exogenous cytokines.
免疫磁珠分选后的细胞亦证实培养刺激对于CD 4 - APC表达的影响很小。
Cells sorted by immunomagnetic beads also confirmed that the stimulation had little effect on the expression of CD4-APC.
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