...T 18979-2003规定了免疫亲和层析净化高效液相色谱检测食品中黄曲霉毒素B1的方法。样品经过甲醇水混合溶液的提取，定量滤纸和玻璃纤维滤纸的过滤，通过免疫亲和柱。免疫亲和柱(IAC)是根据单克隆抗体与载体蛋白偶联后将其填柱形成IAC，并与黄曲霉毒素抗原产生一一对应的特异性吸附关系制作成。
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无标题文档 关键词：伏马毒素；玉 米；免疫亲和柱；高效液相色谱 [gap=1019]Key words ：Fumonisins；corn；Affinity Cartridges；HPLC
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用罗丹明B抗体建立免疫亲和柱(Immunoaffinity Chromatography, IAC)样品净化方法,并利用高效液相-质谱联用(High Performance Liquid Chroma...
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结论：杂交瘤细胞株5e10能稳定的分泌目的单克隆抗体，利用该抗体初步研制的AFB_1免疫亲和柱的性能仍需进一步优化。
Conclusion: the cell line 5e10 could secret the purpose McAb, AFB_1 immunoaffinity column prepared by the McAb needs to be further optimized.
方法：大量样品首先用直接竞争ELISA方法初筛测定，阳性样品再用免疫亲和柱净化、荧光检测器测定的HPLC方法验证和准确定量。
Methods:The concentrations of AFB1 in the samples extracts were initially estimated by ELISA. Samples found to be positive with ELISA were further confirmed and quantified with HPLC.
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