以120份疑似猪瘟病料为试验材料，使用RT-PCR核酸检测与ELISA抗原检测2种方法，分别用这2种方法检测疑似病料，并且比较这2种方法在猪瘟病料检测中的实际差别。
In order to analyze the differences between RT-PCR and ELISA antigen detection methods in classical swine fever virus detection, 120 clinical samples were detected by the two methods.
方法用盐提取法提取人群中白细胞的DNA，以聚合酶链反应、限制性内切核酸酶检测-4533G/A多态性。
Methods DNAs were extracted from the white blood cells of people in Hainan by salt-out method. Polymerase chain reaction and restriction endonuclease was used to determine the 4533G/A polymorphism.
该方法可 以应用于生物检测的许多领域，例如：核酸杂交检测、核酸损伤检测和 化学物质基因毒性的检测。
The invention can be used in biological detection, as nucleic acid cross detection, nucleic hurt detection and chemical material gene toxicity detection.
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