方法：以癌胚抗原(CEA)为分子标记物，采用RTPCR技术检测43例食管癌患者及17例食管良性病变患者肋骨骨髓中癌胚抗原的表达。
Methods: CEA mRNA expression in bone marrow of rib was detected in 43 patients with esophageal carcinoma and 17 patients with esophageal benign lesions as controls by RTPCR.
以辣根过氧化物酶采用改良的过碘酸钠法标记抗体、混合酸酐法标记半抗原。
The horseradish peroxidase (HRP) tagged antibody was prepared by means of modified NaIO 4 method and the HRP tagged hapten was prepared by means of mixed anhydride method.
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