应用寡核苷酸诱导的定点突变和PCR技术对人脑源性神经营养因子基因进行突变，并完成了测序鉴定。

Human brain derived neurotrophic factor gene was used as template for oligonucleotide based site directed mutagenesis and PCR.

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重组人脑源性神经营养因子（BDNF）在大肠杆菌中表达，以冻干形式提供。反相高效液相色谱法和SDS-PAGE测定其纯度大于96％。

Recombinant human BDNF was expressed in E. coli and is supplied in a lyophilized form. A greater than 96% purity was determined by reverse phase-HPLC and SDS-PAGE.

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