目的建立一种人肠系膜小动脉平滑肌细胞急性酶分离法并在分离的细胞上研究其离子通道特性。
Objective To establish an enzymatic isolation method of human mesenteric arteriolar smooth muscle cells and study ion channel characteristics of the isolated cells.
结论：鲎血细胞多肽可以抑制LPS诱导人脐带动脉平滑肌细胞释放NO。
Conclusion: polypeptides from horseshoe crab can inhibit LPS-inducing human umbilical arterial smooth muscle cells secreting NO.
方法：人脐带动脉平滑肌细胞体外原代培养，传至4～ 5代用于实验。
Method:Smooth muscle cell from human umbilical artery was cultured in vitro. 4~5 th generation were took for use.
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