亲和层析法(Affinity Chromatography,AC)是定量分析IgG的最有前景的液相色谱技术之一,其结合了分离和免疫测定特性。
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(2)用途 脱盐和更换缓冲液 、蛋白质分子的分级分离 3、亲和层析法(affinity chromatography,AC) (1)原理 是利用生物高分子能和配基特异、可逆地结合特点,专一地分离纯化生物高分子,又叫选择性...
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镍亲和层析法 HisBind Purification Kit
蛋白A亲和层析法 protein A-sepharose affinity chromatography
前者主要指亲和层析法 affinity chromatography
硼酸亲和层析法 Boric acid affinity chromatography
We isolated the LN receptor from the tissue of Lewis lung carcinoma by affinity chromatography.
本文采用亲和层析法从小鼠Lewis肺癌组织中分离LN受体并对其理化性质进行研究。
参考来源 - 小鼠Lewis肺癌组织中层粘连蛋白受体的分离及其性质的研究After dissolution of inclusion bodies in binding buffer containing 8M Urea, the fusion protein was purified with metal affinity chromatography column, high purity protein was achieved.
包涵体蛋白经含8M尿素的结合缓冲液溶解后,用金属亲和层析法进行纯化,获得了高纯度的目的蛋白。
参考来源 - 马铃薯卷叶病毒CP基因的克隆、原核表达及抗血清的制备·2,447,543篇论文数据,部分数据来源于NoteExpress
抗体采用蛋白a和肽亲和层析法纯化。
Antibodies are purified by protein a and peptide affinity chromatography.
研究了应用金属螯合亲和层析法纯化重组类人胶原蛋白的条件。
Recombinant human-like collagen with a hexahistidine tail attacked to the carboxyl end could be easily purified by Metal Chelated Affinity Chromatography (MCAC).
本文采用亲和层析法纯化的兔抗64DP抗体,建立了简便灵敏的酶免疫测定法(ELISA)。
We have established a rapid and sensitive method-ELISA for quantitative assay of 64DP. 24 samples were parellelly examined by ELISA and rocket electrophoresis.
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