琼脂糖凝胶蛋白芯片片基的制备及应用-资料-中国显微图像网 关键词】 琼脂糖凝胶薄膜 蛋白微阵列芯片 乙肝病毒抗原检测 [gap=5193]Keywords Agarose film, protein microarray chip, hepatitis B virus diagnosis
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材料和方法:对肾移植患者检测出乙肝病毒表面抗原(HBsAg)阳性者进行肝活检,由此确定病毒复制证据。
Patients and METHODS: Renal transplant patients positive for HBV surface antigen (HBsAg) and submitted to a liver biopsy because of evidence of viral replication were included.
方法 用酶联免疫吸附试验对982例乙肝患者血清标志物和乙肝病毒前S2抗原进行检测;并用荧光定量PCR法对其进行HBV-DNA检测。
Methods Hepatitis B serum markers and Pre-S2 antigens were tested by enzyme-linked immunosorbent assay and HBV DNA was detected by fluorescent quantitative-PCR in 982 hepatitis B patients.
结果5例肾组织中检测到乙肝病毒抗原。
Results Hepatitis B virus antigen was detected in kidney tissue of 5 cases.
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