方法：采用流式细胞仪单染色和三色荧光标记技术。
Methods: to use mono color and three color fluorescent staining methods by flow cytometry.
应用三色荧光标记LSCM观察复合种植体周围骨整合情况。
Multiple fluorescent was labeled at different times and then LSCM was used to observe the newly formed bone around the complex implant.
此外，采用流式细胞仪的三色荧光标记检测方法，不仅可以节约D C细胞用量，而且具有快速和准确的优点，值得推广应用。
In addition, by using the method of three fluorescence marker, we could save DC number needed. Moreover this method is fast and exact, which is deserved to be applied.
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