...物5′端带有载体序列编码的若干氨基酸残基,唯有信号肽末端的XhoⅠ位点可以确保表达产物为天然形式,它位于信号肽切割位点(Lys-Arg)的编码序列(AAA AGA)之前,为恢复信号肽的完整性,我们在α-半乳糖苷酶编码序列前端增加这两个氨基酸的编码序列,在下游TGA终止密码...
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通过改变前体mrna的切割位点和聚腺苷酸化位点而从一个编码区产生不同大小mrna的能力。
The ability to make mRNAs of varying sizes from one coding region, by altering the site of pre-mRNA cleavage and polyadenylation.
In this case, this particular restriction enzyme cuts symmetrically like this, but not at the same point.
在我们这种情况下,这个限制性内切酶总是这样对称地切割,但两条链切割的位点不一样
The first step is we had to be able to take this circular DNA and cut it to create a site for our new gene to be added.
第一步是切割环形DNA,以创造出一个整合新基因的位点
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