Results:1. Bone marrow mononuclear cells which were obtained by density gradient centrifugation and NH4CL digestion Contains EPCs,which have endothelial differentiation potential,can achieve vitro expansion of EPCs.2.
结果:1.使用密度梯度离心法和NH4CL消化法获得的骨髓单个核细胞含有具有内皮分化潜能的内皮祖细胞,在适宜的培养条件下可以实现内皮祖细胞的体外扩增。
参考来源 - 糖尿病大鼠骨髓内皮祖细胞体外培养的实验研究·2,447,543篇论文数据，部分数据来源于NoteExpress
ObjectiveTo study the culture technique and differentiation method of lymphatic endothelial cells, and supply support for the related research of this field.
目的探讨淋巴管内皮细胞的培养技术与鉴定方法，为开展有关方面的研究提供技术支持。
In addition, we tested the effect of vessel endothelial growth factor (VEGF) and passage on cell differentiation, expansion kinetics and apoptosis.
另外研究血管内皮生长因子(VEGF)和传代培养对细胞分化、扩增动力学和细胞凋亡的影响。
Objective to study the methods of isolation, culture, differentiation and identification of endothelial progenitor cells (EPC) from peripheral blood.
目的研究外周血来源内皮祖细胞的分离、培养、分化及鉴定方法。
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