The results showed that a certain pulse strength was necessary to achieve embryo fusion, and the duration was a key factor affecting the embryo fusion and its later development.

以上结果表明，一定的脉冲电压强度是胚胎融合的必要条件，而脉冲宽度是影响胚胎融合及其后期发育的关键因素。

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Conclusion Optimal parameters for electrofusion are crucial for the fusion of donor-recipient pairs and the activation of the reconstructed embryo.

结论优化的电融合参数是决定供体核-卵母细胞复合体融合与重组胚激活的关键因素。

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Objective: To explore the best conditions of electrical fusion and activation influencing the development of porcine somatic cell nuclear transfer embryo.

目的：摸索猪体细胞核移植最佳的融合和激活条件。

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