The elution time is a characteristic of an individual compound; and the instrument response, measured as peak area or peak height, is a function of the amount present.
每种组分有其特有的洗脱时间，仪器的响应值，峰面积和峰高都与组分的量有关。
The gradient elution is reasonable when the retention time is 2.3 times after the appearance of prominent peak with initial mobile phase acetonitrile-water (40:60).
用初始流动相乙腈-水(40：6 0)洗脱至主峰出峰保留时间的2.3倍时开始进行梯度洗脱比较合理。
Gradient elution: the configuration of the equipment employed may significantly alter the resolution, retention time and relative retentions described in the method.
梯度洗脱：仪器的配置可能会影响到方法所描述的分离度、保留时间以及相对保留时间。
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