abstract:An ectodomain is the domain of a membrane protein that extends into the extracellular space (the space outside a cell). Ectodomains are usually the parts of proteins that initiate contact with surfaces, which leads to signal transduction.
Objective toclone and construct the plasmid containing human thyroid stimulatinghormonereceptor (TSHR) geneectodomain, and then identify the immunoreactivity of the purifiedrecombinantprotein.