The plasmid was transformed to E. coli BL21(DE3) by screening and identifying to obtain the high expressing positive clone.

再将此等质粒转化至大肠杆菌，经过筛选鉴定获得高表达阳性克隆。

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How easy is it to clone an e-passport?

复制电子护照有多容易？

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Objective To clone and express the Tip operon gene of E. coli and increase the enzyme activity of expressed product.

目的克隆并表达大肠杆菌色氨酸操纵子基因，提高其酶活性。

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The company has moved into the GroupOn clone territory, too, with its own group-buying e-commerce web site Nuomi, which rolled out in June 2010.

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