OBJECTIVE: To reduce the formation of inclusion body in the course of fermentation of E. coli.
目的:减少大肠杆菌发酵过程中包含体的形成。
CONCLUSION: Make use of low temperature induction, the formation of inclusion body had been reduced in the course of fermentation of E. coli.
结论:低温诱导可减少大肠杆菌发酵中包含体的形成。
We determined the content of the total bacterial proteins and the proportion of recombinant porcine growth hormone(rpGH)in inclusion bodies(IBs)in the cultures of genetically engineered rpGH E.
测定了猪生长激素基因工程菌不同表达效率时细菌总蛋白的含量以及包涵体中重组猪生长激素占包涵体总蛋白的比值。
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