Thus, the Tm of PNA–DNA duplex is barely affected by low ionic strength. This significantly facilitates the hybridization with the PNAs.

因此，PNA-DNA 的热熔温度几乎不受弱离子强度的影响，十分有利于PNA的杂交。

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The stability of these triple helixes is so high that homopyrimidine PNA targeted to purine tracts of dsDNA invades the duplex by displacing one of the DNA strands.

这种三螺旋体的稳定性极高，以至于在同型嘧啶PNA靶向双链DNA嘌呤道时，能取代其中一条DNA链侵入双螺旋。

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Objective To investigate the value of duplex polymerase chain reaction (DPCR) in early diagnosis of Legionella pneumonia by detecting Legionella DNA in sputum and bronchoalvelar lavage fluid(BALF).

目的探讨双重聚合酶链反应（DPCR）法检测痰及支气管肺泡灌洗液（BALF）中军团菌DNA在早期诊断军团菌肺炎的意义。

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