The mutant G138P was obtained by in vitro site directed mutagenesis of GI gene.
用双引物法对GI基因进行体外定点突变,构建了GI突变体g 138p。
Site-directed mutagenesis is one of the key methods used to study gene function.
定点突变是研究基因功能的一种关键方法。
Objective: To develop a simple and efficient way to perform site-directed mutagenesis.
目的:介绍一种简便、有效的定点突变技术。
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