Fusarium moniliforme CGMCC 0536;D-lactonohydrolase;cDNA;Cloning;Expression;Protein engineering;Error prone PCR;DNA shuffling;Screening;Directed evolution.
串珠镰孢霉菌;D-泛解酸内酯水解酶;克隆;表达;分泌;大肠杆菌;酿酒酵母;易错PCR;DNA改组;定向进化;筛选。
参考来源 - DIn this study,inteins with low splicing activity were evolved with directed evolution strategy. The aim is to obtain more applicable inteins with high protein splicing activity.
本研究以具有极低剪接活性的蛋白质内含子为实验材料,利用定向进化策略,最终达到提高其在异源宿主中剪接活性的目的。
参考来源 - 异源宿主中蛋白质内含子剪接活性的定向进化Developing a high-throughput screening method is of great importance for directed evolution of atrazine chlorohydrolase.
阿特拉津氯水解酶定向改造的关键是开发一种廉价的、表型改变明显的高通量筛选方法。
参考来源 - 利用雨生红球藻表达系统高通量筛选活力提高的阿特拉津氯水解酶突变子·2,447,543篇论文数据，部分数据来源于NoteExpress
以上来源于: WordNet
Then it would be autonomous evolution instead of directed evolution.
之后，取代定向进化的就将是自发的进化。
Directed evolution is a new powerful strategy for engineering proteins.
定向进化是改造蛋白质分子的一种有效的新策略。
Directed evolution has been widely used in the studying of modifying or enhancing protein performance.
定向进化已经被广泛用于修饰和加强蛋白质性能的研究。
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