Methods Molecular biology technologies of RT-PCR, directed cloning and DNA sequencing were carried out.

方法采用RT -PCR、定向克隆和DNA测序等一系列分子生物学技术进行本实验。

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Here, we summarize our recent progress using directed RT-PCR cloning and DNA synthesis.

这里，我们使用定向rt - PCR克隆和DNA合成概述了我们最新的进展。

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