⑴PCR产物直接测序（direct PCR sequencing）：该技术一次可准确测序HBV逆转录酶A～E区的全长549bp。可通过一次实验，检测全部已知和可能的耐药变异位点。
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DNA Direct PCR-sequencing method DNA直接测序法
direct pcr product sequencing 直接测序
pcr product direct sequencing 聚合酶链反应产物直接测序
direct sequencing of pcr product pcr产物直接测序法
Mutation analysis was detected by direct sequencing RT-PCR products.
直接测序法检测RT PCR产物的基因变异；
CONCLUSION: We first amplified mtDNA in circulating plasma from breast cancer patients by direct PCR, checked mutation by DHPLC and confirmed results by sequencing.
结论：本实验直接用PCR扩增乳腺癌血浆核酸中线粒体基因，并用DHPLC初步筛选突变并测序证实。
METHODS ITS1 and ITS2 genes of Pogostemon Cablin from different localities were identified by PCR direct sequencing.
方法采用PCR直接测序技术对不同产地的广藿香its1和ITS2基因进行测序分析。
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