A HPLC-MS method was applied to determine the metabolites formation of six CYP450s probe substrates(phenacetin-CYP1A2,dextromethorphan-CYP2D2,diclofenac sodium-CYP2C6,mephenytoin-CYP2C11,chlorzoxazone-CYP2E1 and midazolam-CYP3A1/2) in rat liver microsomal incubations. The activity of CYP450 isozymes were represented by the formation of metabolites.
串联质谱分析方法测定CYP450的6种同工酶特异性探针底物非那西丁(CYP1A2)、右美沙芬(CYP2D2)、双氯芬酸钠(CYP2C6)、美芬妥英(CYP2C11)、氯唑沙宗(CYP2E1)、咪达唑仑(CYP3A1/2)在大鼠肝微粒体的代谢产物生成(对乙酰氨基酚、右啡烷、4-羟基双氯芬酸钠、4-羟基美芬妥英、6-羟基氯唑沙宗、1-羟基咪达唑仑)以反映各CYP450同工酶活性。
参考来源 - 混合探针底物法测定五味子成分对大鼠肝脏细胞色素P450同工酶的影响·2,447,543篇论文数据，部分数据来源于NoteExpress
应用推荐