Due to ubiquitous repeats in large genomes and the inability of short sequences to uniquely and unambiguously characterize them, the short read length limits applicability for denovo sequencing.
由于在大的基因组中广泛存在着重复，短序列不能惟一明确的表征它们，短长度限制了从头测序的适用性。
Our study shows quite clearly that this approach solely using 454 Systems gives excellent results for large genome sequencing and denovo assembly projects.
Jakobsen教授说：“我们的研究很清楚地表明，只使用454系统已经完成大基因组的测序以及优质的全新基因组拼接。”
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