珍稀药用金钗石斛组培苗遗传稳定性直接扩增长度多态性检测-药学论文-中国论文联盟 关键词】 金钗石斛; 组培苗; 直接扩增长度多态性; 遗传稳定性; 指纹图谱 [gap=867]Key words:Dendrobium nobile; Tissue culture seedling; DALP; Genetic stability; Fingerprinting
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Objective To establish a set of stable DALP reaction system of Stephania species and set foundation for DALP molecular markers of this genus.
目的建立一套稳定的千金藤植物DALP反应体系,为开展该属植物的DALP分子标记奠定基础。
Methods Some significant parameters of DALP reaction procedure were investigated and optimized by taking the DNA genome for the plants of Rhodiola L. as template.
方法以红景天基因组dna为模板,对DALP反应程序的一些重要参数进行摸索和优化试验。
Objective Direct amplification of length polymorphism (DALP) as a new molecular marker was used to establish a set of stable DALP reaction system for the plants of Rhodiola l.
目的利用直接扩增片段长度多态(DALP)这一新分子标记技术建立一套稳定的红景天属植物的DALP反应体系。
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