AIM: to construct the recombinant expression vector and to obtain large amount of mouse Cyclin D1 protein fused to PTD.
目的：构建融合重组表达载体并对其进行诱导表达和蛋白纯化以获得大量重组融合蛋白。
Conclusion Ribozyme targeting cyclin Dl may effectively inhibit cyclin D1 expression and activation in the HSC T6 cell line.
结论载体表达的特异性核酶能有效地抑制HSC的细胞周期蛋白d1的表达和激活。
Both clones included a DNA sequence that allow a fluorescent protein expressed in the cell to bind to cyclin D1 RNA the moment it is transcribed.
这两个克隆都包含有一段DNA序列，能在在细胞周期蛋白d1的RNA被转录的时候将荧光蛋白绑定到其上。
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