Cryostat sections were cut coronally through the hippocampi of the brains at weeks 2, 4, 8 and 12 after transplantation respectively.
分别在移植后第2、4、8、12周4个时间点取脑冷冻切片，在倒置荧光显微镜下观察移植细胞的存活和迁移。
Methods After perfused fixation and quick freezing, the tissues of whole CNS were cut into serial sections with cryostat followed by hematoxylin stain.
方法经过灌流固定的CNS组织低温冰箱速冻后连续冰冻切片和改良的苏木精染色方法。
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