Subsequently competent cells were prepared and transformed. After large-scale preparation of plasmid DNA, recombined adenoviruses Ad5F35-IL24 were generated with homologous recombination by cotransfection of 293 cells with a couple plasmids.
制备感受态细胞,进行转化,大量制备质粒DNA;之后用双质粒共转染293细胞,产生重组腺病毒Ad5F35-IL24。
参考来源 - 腺病毒介导IL·2,447,543篇论文数据,部分数据来源于NoteExpress
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