The effects of mobile phase composition, flow rate, column temperature, gradient ramp, ph value, salt concentration, and buffer concentration on capacity factors (K ') were respectively discussed.
对流动相组成、流率、柱温、梯度速率、pH值、盐浓度以及缓冲液浓度对容量因子(K ')的影响分别进行了讨论。
METHODS: Searching for gradient profile and column temperature by grid rake techniques on different band C-18 columns and HPLC instruments.
方法：在不同品牌的HPLC仪器和十八烷基硅烷键合硅胶色谱柱上，对梯度条件和柱温进行网格搜索。
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