Two parts were included in the studies:Part one : Cloning and expression of hemolytic-toxin from Actinobacillus pleuropneumoniae.
本研究内容分两个部分:第一部分:猪胸膜肺炎放线杆菌溶血毒素的克隆与表达。
参考来源 - 猪胸膜肺炎放线杆菌溶血毒素的克隆表达及表达产物对小白鼠的免疫保护性研究The second part is the cloning and expression of the DJ-1 encoding sequence.
第二部分为大鼠DJ-1的克隆与表达。
参考来源 - 大鼠DJObjective Cloning and expression a new serine protease with fibrinolytic activity from cDNA library established from clamworm digestive tract epithelial cells by RACE technology and detecting its bio-activity using artificial fibrin plates.
①目的：从沙蚕上皮细胞的cDNA文库中克隆和表达具有纤溶活性的一种新型的丝氨酸蛋白酶。
参考来源 - Ⅰ：沙蚕纤溶酶—沙蚕激酶的克隆、表达及活性测定 Ⅱ：汉滩病毒截短核蛋白的表达和初步应用LY822, and the cloning and expression of its dsz genes were investigated. The results preliminary clarified the biodesulfurization metabolism and verified the sulfur-starvation-induced mechanism of desulfurization enzyme formation in Rhodococcus sp.
本论文以脱硫菌Rhodococcus sp．LY822为出发菌株，开展了硫酸盐对LY822菌脱硫代谢的影响、脱硫基因克隆表达及重组菌脱硫活性的研究，初步阐明了脱硫代谢机理，验证了脱硫酶生成的“硫饥饿”诱导机制。
参考来源 - 硫酸盐对Rhodococcus sp. LY822脱硫代谢影响的研究及脱硫基因的克隆表达·2,447,543篇论文数据，部分数据来源于NoteExpress
Cloning and expression of N-acetyl-D-glucosamine2-epimerase.
乙酰-D-葡萄糖胺2-差向异构酶基因的克隆及表达。
Purpose Molecular cloning and expression of pro nattokinase gene with e.
目的构建纳豆激酶原基因克隆，实现其在大肠杆菌中的表达。
The gene structure of antibacterial peptides and the research of cloning and expression of the genes are .
本文主要综述了抗菌肽基因的克隆与表达的研究进展并展望了抗菌肽在畜牧业生产中的应用前景。
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