Hepatic stellate cell was isolated with collagenase perfusion and ladder density centrifuge. Hepatic stellate cell line IG12 was cloned with limited diluted method.
利用胶原酶灌注梯密度离心分离肝星状细胞，用有限稀释法建立大鼠肝星状细胞株ig12。
Conclusion HumanPD-1 gene has been cloned, recombinant retrovirus vector containing PD-1 gene constructed and L929 transfection cell line expressing PD-1 molecules selected.
结论构建了含人pd - 1基因重组逆转录病毒的载体，筛选出稳定表达人PD - 1分子的L929细胞株。
应用推荐