Objective: To clone and express the gene of human enterokinase light chain which would be used in the cleavage and purification of fusion proteins.
研究目的：克隆表达人肠激酶轻链编码基因，以期应用于融合蛋白的切割与纯化。
Purification of Milli-Q water by sub-boiling distilled and employment of qualified FBS could increase the AKP staining positive level and total clone formation rate of mouse ES cells.
Q 水经亚沸处理能显著提高ES细胞保持未分化状态的能力，优质胎牛血清能提高ES的AKP阳性度和克隆形成率。
Results and Conclusion: the PCR screening method was convenient and fast for confirming positive recombinant clone, and there was no need for preparation and purification of the plasmid.
结果和结论：以pcr方法筛查重组阳性克隆，可以简便快速鉴定重组阳性克隆，不需提取质粒。
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