The dioscin was used as the control sample, the vanillin-perchloric acid as chromogenic agent, glacial acetic acid as solvent system at 60Cfor 15min. Then,the content of total saponins in lilium brownii were determined by spectrophotometry at 540nm.
以薯蓣皂甙为对照品，香草醛-高氯酸为显色剂，冰醋酸为溶剂，60℃恒温15min，分光光度法测定百合总皂甙的含量。
参考来源 - 百合皂甙的提取、纯化及其对羟自由基清除作用的研究·2,447,543篇论文数据，部分数据来源于NoteExpress
Optimum measuring wave length was selected. The effect of acidity, concentration of citric acid and chromogenic agent, temperature and interfering elements on the measurement was studied.
优选出最佳测量波长，研究了酸度、柠檬酸浓度、显色剂浓度、温度及干扰元素对测量的影响。
Rhodium in rhodium plating bath was determined by spectrophotometric method using potassium iodine and stannous chloride as chromogenic agent .
以碘化钾和氯化亚锡作显色剂，用光度法测定镀铑溶液中的铑。
Copper sulfate in acid copper plating bath was determined by spectrophotometry with EDTA as chromogenic agent.
用EDTA作显色剂，通过光度法测定酸性镀铜液中硫酸铜的含量。
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