... centro dogma 释义：中心法制 centrifuging method 释义：离心法 cesium chloride gradient centrifugation 释义：氯化铯梯度离心法 ...
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Methods:Myeloid cells of Hindlimb bone were collected in Wistar male rat at the age of 4 weeks,weighting 100～120g in asepsis condition. BMSCs were isolated by gradient centrifuging method and adherent culture in vitro.
方法:在无菌条件下取四周龄100～120g健康雄性Wistar大鼠双后肢的骨髓细胞,采用密度梯度离心法与贴壁培养相结合的方法,体外分离BMSCs。
参考来源 - 大鼠骨髓间充质干细胞的生物学特性及其对急性化学性肝损伤的修复作用·2,447,543篇论文数据，部分数据来源于NoteExpress
This method contains steps as follows: pre-processing raw material, adding extracting agent which contains ionic liquid, centrifuging, filtering, purifying and condensing the extracting liquid.
该方法包括下列步骤：原料预处理，加入含离子液体的萃取剂，萃取液离心、过滤、纯化、浓缩即可。
Methods: Routine method, medium diluting method and centrifuging and filtering method were used for the verification of methodology on 5 antipyretics.
方法：采用常规法、培养基稀释法、离心集菌薄膜过滤法对五种解热镇痛类药进行方法验证。
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