METHODS: MTT assay to test cytotoxicity; cell staining and NBT reduction to test cell differentiation.
方法:MTT法测定细胞增殖,细胞染色和NBT还原试验测定细胞分化。
METHODS: We used RT-PCR, flow cytometry and immunofluorescence cell staining techniques to observe the expression of PARs on A549 cells.
方法:采用逆转录聚合酶链反应(RT - PCR)、流式细胞仪和免疫荧光细胞染色分析技术分析肺上皮细胞系a549细胞PAR s的表达情况。
Staining of a thin sliver of each block can highlight the main bodies of nerve cells (top, purplish image) with the darker areas indicating high cell density near the brain's surface.
对每一块的细微纹路进行着色能突显出神经细胞的主体部分(上面略带紫色的图)。图中的偏暗区域代表了大脑表明的高细胞密度区域。
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