Results Homologous integration of SV40T gene into RAE - 1 cell genome was positively detected by PCR. More than 95% cells were proved positively by alkaline phosphatase staining.
结果转染后成骨细胞的基因组中整合有SV40T基因,碱性磷酸酶染色阳性细胞占95%以上;
They lie outside the nucleus that houses the main human genome, and because there may be hundreds of them in each cell, their DNA is particularly easy to detect.
它们位于人类基因组的核心之外,由于在每个细胞内它们的存在都数以百千计,因此它们的DNA就特别容易被检测到。
After these genomes are synthesized, the first step is just transplanting them into a cell without a genome.
这些基因组合成之后,第一步就是移植到另一个没有基因组的细胞里。
But first,Mister Venter said, they had to de-activate a gene in the receiving cell that would have killed the synthetic genome.
VOA: special.2010.07.20
They integrate their DNA into the host genome and you might not want to make that kind of a change in the cell.
而且也许你也并不想让逆转录病毒基因,整合进宿主的基因组
Out of all the genes that are on the human genome which fraction is this particular cell using, which fraction is it expressing determines what proteins are present in the cell, determines what work or what activities the cell can engage in.
人类基因组全部基因中的,特定细胞所表达的基因片段,细胞表达的是哪段基因,决定了细胞中存在什么样的蛋白,决定了细胞会有什么样的功能,或者参与何种生命活动
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