Methods MBP-specific T lymphocytes were established from C57BL/6 mouse cultured in vitro and stimulated with MBP and allogenic APCs for several times.
方法取MBP免疫的C57BL／6 小鼠的淋巴细胞在体外培养，用MBP及同系小鼠的脾细胞反复刺激，建立了MBP- 特异性T淋巴细胞。
METHODS: Mouse lymphoma EL4 cells were inoculated subcutaneously into wild type C57BL/6 mice (immune-competent mice).
方法：给正常野生型C57BL/6小鼠皮下接种小鼠淋巴瘤EL4细胞，建立荷EL4肿瘤的小鼠模型。
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