But once passed, it is gone forever.

而时间一旦度过，便永远地一去不返。

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But fears that once handled in a supermarket the bacteria can be passed on through trolley handles, shopping bags and transfered to other foods.

不过他们担心一旦进入超市出售，这些细菌就会通过购物车手柄、购物袋传播至其他食品。

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Many hours have passed and my only company was "just a dog," but I did not once feel slighted.

在仅有“区区一只狗”陪伴的数小时里，我从未感到过被忽视。

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But once Congress passed it, Southern politicians, citizens and institutions quickly fell in line.

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But once it passed, in 2009, she was impressed with its impact.

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Investors would be given time to redeem, but once the deadline passed, their assets would be locked inside the fund.

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