In order to clarify these controversial findings, we developed a new bisulphite sequencing protocol to determine the methylation status of p16.
为了阐明这些有争议的发现，我们采用了一种新的亚硫氰酸盐测序工具来确定。
Bisulphite conversion of unmethylated cytosine residues followed by detection methods such as sequencing of distinct loci has become accepted as the gold standard for detecting 5-methylcytosines.
非甲基化胞嘧啶残基的亚硫酸氢盐转换，接着是检测方法，例如不同位点的测序已经被接受为检测5甲基胞嘧啶的金标准。
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