The column must be pre-equilibrated in binding buffer before beginning sample application.
列必须在开端抽样应用之前在捆绑的缓冲器中被平衡。
Gets or sets the maximum amount of memory allocated for the buffer manager that manages the buffers required by endpoints using this binding.
获取或设置可为缓冲区管理器分配的最大内存量，该管理器管理使用此绑定的终结点所需的缓冲区。
We furthermore found that an appropriate buffer ion can intensify the binding although its concentration and acidity remain constant (8).
我们进一步发现适合的缓冲离子能强化结合，虽然它的浓度和酸度都保持不变。
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