Firstly, the processing conditions of batch culture of recombinant bacteria in 5L fermenter, including whether adding trace element and vitamin to culture media and so on were optimized.
首先在5L发酵罐中，对工程菌DH5α／pBV220间歇培养时，是否在培养基中添加微量元素和维生素等工艺条件进行了优化，确定出间歇培养的最优培养条件。
参考来源 - 重组人粒细胞集落刺激因子生产工艺的研究In fed-batch culture, the best fed volum was 60% of the working volum, the best time was peak time of hydrogen production, in above conditions, YUAN-3 kept higher growth and hydrogen-producing activity.
补料培养的最佳补料体积为发酵液体积的60%左右,最佳的补料时间为产气的高峰期,在此条件下投加细菌可以保证细菌具有较高的生长和产氢活性,并可以缩短延滞期。 连续培养能够不断将发酵产物排出,引进新鲜培养基,避免底物的抑制。
参考来源 - 自凝集产氢细菌代谢特性及扩大培养研究There were no obvious lag stage in batch culture of Artemisia annua L hairy root,7～15 days after inoculation was the exponential stage,the growth rate of 11th day was the highest,and the cultures reached stationary stage at 20th day.
青蒿发根在分批培养中没有明显的迟滞期，接种后第7天进入指数生长期，第11天生长最快，第20天进入稳定期。
参考来源 - 青蒿发根生长及青蒿素生物合成动态的研究·2,447,543篇论文数据，部分数据来源于NoteExpress
The theory of metabolic engineering was applied to a fed batch culture of animal cells.
将代谢工程理论应用于动物细胞的流加培养过程。
The research in the sift of kinds fit to batch culture and the main ecological factor have been completed.
完成了适宜批量培养的种类的筛选及主要生态学因子的研究工作。
Microbial cultivation methods can be classified into batch culture, semi-continuous culture and continuous culture.
微生物的培养方式分为分批培养、半连续培养和连续培养，其中，分批培养应用最为广泛。
应用推荐