During the operations, sampling and bacteria counting were taken before the first operation and after the second one.
分别在第一台手术前、第二台手术毕进行采样培养，进行细菌计数。
Freeze dry process that BCG live bacteria counting after 4 ~ 5 years after storage remained at the level of conformity.
冻干工艺使卡介苗的活菌数经4 ~ 5年存放后仍保持在合格水平。
Cellulose-decomposed bacteria and methanogen counting decreased, but the effect of alfalfa saponin on methanogen counting was more than on cellulose-decomposed bacteria counting.
添加苜蓿皂甙对细菌总数的影响不大但对纤维分解菌和产甲烷菌的生长和繁殖有抑制作用，对产甲烷菌的影响大于对纤维分解菌的影响。
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