Use a total internal reflection fluorescence microscope to observe, the latter can be sent to filter out the background fluorescence.
这里用全内反射荧光显微镜来进行观察，就可以把后者发出的背景荧光过滤掉。
By photon-burst mothod, the fluorescence photons of dye molecules can be distinguished from the background reflecting photons.
通过探测“光子爆发”，把单个染料分子的荧光同很强的背景杂散光区别开来。
In order to eliminate or reduces the background or fluorescence influence, it is better to operate in the darkroom or the dark cover screening, to completely eliminate the environment light influence.
为了消除外界背景或减小荧光的影响，最好还是应该在暗室或暗罩遮护中进行，以完全消除环境光的影响。
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