...要:目的:(1)以单细胞细菌人工染色体一荧光原位杂交(bacterialartificialchromosome-Fluorescentinsituhybridisation,BAC-FISH)为核心技术,建立染色体易位携带者植入前遗传学诊断(preimplantationgeneticdia..
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...要:目的:(1)以单细胞细菌人工染色体一荧光原位杂交(bacterialartificialchromosome-Fluorescentinsituhybridisation,BAC-FISH)为核心技术,建立染色体易位携带者植入前遗传学诊断(preimplantationgeneticdia..
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The results demonstrated a important factor of receiving specific signals of BAC-FISH by select maize BAC-probes for lesser content repeat sequences.
同时,验证了选择重复序列含量较少的玉米BAC作为FISH杂交的探针也是获得特异性杂交信号的重要条件。
The above BAC clones of 20 chicken genes were hybridized by FISH with duck and ostrich metaphase chromosomes prepared from embryo fibroblasts.
将上述定位的鸡的20个功能基因的BAC克隆分别与利用胚胎成纤维细胞制备的鸭和鸵鸟的中期染色体杂交。
BAC clones corresponding to telomeres, as well as to the centromere position and the gap sizes between contigs, were determined by BAC-pachytene chromosome fluorescence in situ hybridization (FISH).
端粒克隆、着丝粒克隆以及重叠群之间空缺的大小都经过粗线期染色体荧光原位杂交鉴定。
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